Method for Printing Functional Protein Microarrays
نویسندگان
چکیده
منابع مشابه
Short Technical Report Method for Printing Functional Protein Microarrays
Piezoelectric dispensing of proteins from borosilicate glass capillaries is a popular method of protein biochip fabrication that offers the advantages of sample recovery and noncontact with the printing substrate. However, little regard has been given to the quantitative aspects of dispensing minute volumes (1 nL or less) at the low protein concentrations (20 μg/mL or less) typically used in mi...
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Supporting Information Proteins were from Sigma and used as received. Antibodies were labelled either with tetramethylrhodamine isothiocyanate (TRITC) or fluorescein isothiocyanate (FITC). Solutions of proteins and adsorption experiments were done in PBS or in PBS-containing 1% BSA. Bis(sulfosuccinimidyl)suberate (BS 3) was from Pierce, aminopropyltriethoxysilane (APTS) from Fluka. PDMS stamps ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03342mt02